Skip Navigation

JNCI Journal of the National Cancer Institute 1992 84(9):699-703; doi:10.1093/jnci/84.9.699
© 1992 by Oxford University Press
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Xing, P.-X.
Right arrow Articles by McKenzie, I. F.C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xing, P.-X.
Right arrow Articles by McKenzie, I. F.C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of the National Cancer Institute, Vol. 84, No. 9, 699-703, May 6, 1992
© 1992 Oxford University Press

Second-Generation Monoclonal Antibodies to Intestinal MUC2 Peptide Reactive With Colon Cancer

Pei-Xiang Xing, Julie Prenzoska, Guy T. Layton, Peter L. Devine, Ian F.C. McKenzie*,

Austin Research Institute, Austin Hospital Heidelberg, Victoria, Australia
Medical Innovations Limited Labrador, Queesland, Australia

*Correspondence to: Ian F. C McKenzie, M.D., Phd., Austin Research Institute, Austin Hospital, Kronheimer Bldg., Studley Rd., Heidelberg, 3084 Victoria, Auatralia.

Background: Antitumor antibodies have traditionally been made to whole tumors or tumor extract. The use of defined synthetic antigens would be desirable for producing monoclonal antibodies. Purpose: Our purpose was to determine if antipeptide antibody to MUC2 had antitumor activity and specificity. Methods: A 29aminoacid peptide to MUC2 was synthesized and monoclonal antibodies were produced after immunizing BALB/c mice with peptidekeyholelimpet hemocyanin in complete Freund's adjuvant, and the monoclonal antibodies were tested on peptides and human tissues. Results: CCP31, CCP37, and CCP58 monoclonal antibodies were produced using MUC2 MI29 (KYPTTTPISTTTMVTPTPTPTGTQTPTTT) containing one repeat unit of 23 amino acids and part of the next repeat of four amino acids. These antibodies reacted with the MUC2derived peptide but not with MUC1 or MUC3derived peptides. One of the monoclonal antibodies, CCP58, reacted strongly with human colon cancer and normal intestine in both fresh and formalinfixed tissues; two other antibodies, CCP37 and CCP31, reacted only with fresh human tissues of normal colon and malignant colon tumors by immunoperoxidase staining. In addition, CCP37 and CCP58 reacted strongly with human gastric cancer; all antibodies reacted weakly with human salivary gland, and none reacted with tissues from normal human lung, kidney, stomach, pancreas, or endometrium. By analysis of mucin molecules by Western blotting, the antigen detected by monoclonal antibodies CCP37 and CCP58 was found to be of a high relative molecular mass (520 kd). Conclusions: AntiMUC2 peptide antibodies appear to be relatively tissue specific and represent a new method of producing antitumor antibodies. [J Natl Cancer Inst 84:699–703,1992


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Clin. Pathol.Home page
A S Mall
Analysis of mucins: role in laboratory diagnosis
J. Clin. Pathol., September 1, 2008; 61(9): 1018 - 1024.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
R A O'Donnell, A Richter, J Ward, G Angco, A Mehta, K Rousseau, D M Swallow, S T Holgate, R Djukanovic, D E Davies, et al.
Expression of ErbB receptors and mucins in the airways of long term current smokers
Thorax, December 1, 2004; 59(12): 1032 - 1040.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Mesquita, N. Jonckheere, R. Almeida, M.-P. Ducourouble, J. Serpa, E. Silva, P. Pigny, F. S. Silva, C. Reis, D. Silberg, et al.
Human MUC2 Mucin Gene Is Transcriptionally Regulated by Cdx Homeodomain Proteins in Gastrointestinal Carcinoma Cell Lines
J. Biol. Chem., December 19, 2003; 278(51): 51549 - 51556.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
R-Q Wang and D-C Fang
Alterations of MUC1 and MUC3 expression in gastric carcinoma: relevance to patient clinicopathological features
J. Clin. Pathol., May 1, 2003; 56(5): 378 - 384.
[Abstract] [Full Text] [PDF]

Home page
 Jpn J Clin OncolHome page
H. Feng, M. Ghazizadeh, H. Konishi, and T. Araki
Expression of MUC1 and MUC2 Mucin Gene Products in Human Ovarian Carcinomas
Jpn. J. Clin. Oncol., December 1, 2002; 32(12): 525 - 529.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Medina, D. Velez, J. A. Asenjo, G. Egea, F. X. Real, J. Gil, and J. L. Subiza
Human Colon Adenocarcinomas Express a MUC1-associated Novel Carbohydrate Epitope on Core Mucin Glycans Defined by a Monoclonal Antibody (A10) Raised against Murine Ehrlich Tumor Cells
Cancer Res., March 1, 1999; 59(5): 1061 - 1070.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Bergeron, S. Champetier, Hélèn. LaRue, and Y. Fradet
MAUB Is a New Mucin Antigen Associated with Bladder Cancer
J. Biol. Chem., March 22, 1996; 271(12): 6933 - 6940.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.