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JNCI Journal of the National Cancer Institute 1992 84(9):690-693; doi:10.1093/jnci/84.9.690
© 1992 by Oxford University Press
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Journal of the National Cancer Institute, Vol. 84, No. 9, 690-693, May 6, 1992
© 1992 Oxford University Press

Expression of Myeloid Cell Phenotypes by a Novel Adult T-Cell Leukemia/Lymphoma Cell Line

Shigeki Koziumi*,, Michiyo Iwanaga, Shosuke Imai, Katsushige Yamashiro, Chikara Mikuni, Shuichi Abe, Mitsuaki Kakinuma, Nagahito Saito, Tsuyoshi Takami, Yuichiro Fukazawa, Masanao Miwa, Toyoro Osato

Department of Virology, Cancer Institute. School of Medicine, Hokkaido University Sapporo, Japan
Chromosome Research Unit, Faculty of Science., Hokkaido University Sapporo, Japan
Section of Bacterialk Infection. Institute of Immunological ScienceHokkaido University Sapporo, Japan
Third Department of Internal., Medicine Hokkaido University Sapporo, Japan
National Sapporo Hospital Sapporo-Japan
Second Department of Pathology, Gifu University School of Medicine Gifu, Japan
Sapporo City General Hospital Sapporo, Japan
National Cancer Center Research Institue Tokyo, Japan
Present address: Biochemistry Department. The University of Tsukuba School of Medicine. Tsukuba. Japan.

*Correspondence to: Shigeki Koizumi, M.D., Ph.D., Department of Virology, Cancer Insitute, Hokkaido University School of Medicine, Sapporo 060.N15, Japan

Background: Human T-cell leukemia virus type 1 (HTLV-1)can infect a number of cells of different lineages in vitro. yet the immunophenotypes of most aduly T-cell leukemia/lymphomas (ATLs) are restricted to CD4³. The apparent discrepancy between these findings is still largely unknown.Purpose: We report on a unique case of ATL in which the leukemia cells were positive for both T-cell and myeloid cell antigens. To characterize these cells, we isolasted cell lines from this patient with ATL. Methods: The fresh leukemia cells were cultured without the addition of interleukin-2. Cell cloning was carried out by limiting dilution. Results: A cell line (MU) and its clonasl sublines were established. Mu cells showed the same chromosomal abnormalities and T-cell receptor beta-chain gene rearrangement pattern as those of fresh leukemia cells. MU cells were exclusively positive for a myeloid cell marker (CD13) but not for T-cell markers, despite the presence of T-cell receptor gene rearrangement. Conclusion: The established ATL cell line showed both T-cell and myeloid cell characteristics, which seems to be the first evidence for the close association of ATL cells with both lymphoid and myeloid features. The cell line may provide a new insight for the targets of HTLV-1 infection and transformation in vivo. [J Nastl Cancer Inst 84:690–693, 1992]


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