© 1992 by Oxford University Press
Journal of the National Cancer Institute, Vol. 84, No. 7, 506-510,
April 1992
© 1992 Oxford University Press
Heterogeneity for Allelic Loss in Human Breast Cancer
Geraldine Brush Cancer Research Institute at California Pacific Medical Center San Franciso, Calif.
School of Medicine, University of California San Francisco
Biomedical Science Division, Lawrence Livermore Laboratory Livermore, Calif
*Correspondence to: Helene S. Smith, Ph.D., Geraldine Brush Cancer Research Institute at California Pacific Medical Center, San Franciso, Calif, CA 94115.
Background: Loss of heterozygosity (LOH) at specific chromosomal regions in the tumor cells implicates the presence of tumor suppressor genes. However, it is also possible for an LOH to be randomly acquired and irrelevant to tumor development. Purpose:To determine whether a particular LOH in human breast carcinomas represents a loss of tumor suppressor gene or merely a random loss, we analyzed untreated primary breast cancers for LOH. Methods: Ninety-eight primary human breast cancers from previously untreated patients were analyzed for LOH at 12 chromosomal regions including five randomly selected regions and seven regions previously reported in other cancer types and/or breast cancers. Results: The baseline incidence of LOH was five out of 124 tests (4%) using randomly selected probes on chromosomes 1p, 2p, 4p, 11q, and Xq. Incidences of LOH significantly greater than background were seen in the following chromosomal regions: 22q (10 of 26 cases, 38%); 18p (five of 24 cases, 21%); 17p (30 of 59 cases, 51%); 13q (five of 14 cases, 36%); 3p (13 of 28 cases, 46%); and 1q (18 of 70 cases, 26%). In contrast to previous reports, the incidence of LOH was not significantly different from background for 11p15. In all cases, results were the same for metastatic lymph nodes and primary tumors, suggesting that the losses occurred early in the malignant progression. In cases with LOH at more than one locus, the same DNA sample often varied in degree of signal reduction for the missing alleles. Conclusion: These observations indicate the presence of both intrtumor and intratumor heterogeneity for LOH. [J Natl Cancer Inst 84: 506–510, 1992]
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