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JNCI Journal of the National Cancer Institute 1991 83(17):1218-1225; doi:10.1093/jnci/83.17.1218
© 1991 by Oxford University Press
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Journal of the National Cancer Institute, Vol. 83, No. 17, 1218-1225, September 4, 1991
© 1991 Oxford University Press

Photodynamic Killing of Human Squamous Cell Carcinoma Cells Using a Monoclonal Antibody-Photosensitizer Conjugate

Frank N. Jiang1, Daniel J. Liu2, Herma Neyndorff2, Michael Chester2, Shi-yi Jiang3, Julia G. Levy*,4

1Department of Microbiology, University of British Columbia Vancouver, British Columbia. Canada
2Quadra Logic Technologies, Inc. Vancouver, British Columbia, Canada
3Department of Chemistry, Shanghai Medical University People's Republic of China
4Department of Microbiology, University of British Columbia Vancouver, British Columbia. Canada

*Correspondence to: Julia G. Levy. M.D.,Department of Microbiology #300, 6174 University Blvd., Vancouver, BC, V6T 1Z3, Canada

We have developed procedures in which the photosensitizer benzoporphyrin derivative monoacid ring A (BPD) can be covalently linked to carrier molecules of modified polyvinyl alcohol (PVA) to produce water-soluble PVA-BPD conjugates with a molecular mass in the range of 30 kd. These carriers can subsequently be covalently linked to monoclonal antibodies (MoAbs) using heterobifunctional linking agents. We describe here such a conjugate in which the MoAb (5E8) has specificity for a glycoprotein detected on human squamous cell carcinomas of the lung. We provide evidence that the conjugates produced were covalently linked and retained both their photosensitizing and antigen-binding activities. We show further that the MoAb-PVA-BPD conjugate, in the presence of 10% fetal calf serum, exhibited highly enhanced phototoxic killing of the target cell line (A549) over that exhibited by free BPD or a control MoAb-PVA-BPD conjugate. These results demonstrate, therefore, both the selectivity and specificity of this MoAb conjugate. [J Natl Cancer Inst 83:1218–1225, 1991]


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