© 1990 by Oxford University Press
Journal of the National Cancer Institute, Vol. 82, No. 13, 1107-1112,
July 4, 1990
© 1990 Oxford University Press
New Colorimetric Cytotoxicity Assay for Anticancer-Drug Screening
Developmental Therapeutics Program, Division of Cancer Treatment
Program Resources, Inc., National Cancer Institute, Frederick Cancer Research Facility Frederick, MD.
Developmental Therapeutics Program, Division of Cancer Treatment, National Cancer Institute Bethesda, MD.
*Correspondence to: Philip Skehan, M.D., Developmental Therapeutics Program, Division of Cancer Treatment, Bldg. 560, Rm. 3260, National Cancer Institute, Frederick Cancer Research Facility, Frederick, MD 21701
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the National Cancer Institute's disease-oriented in vitro anticancer-drug discovery screen, which requires the use of several million culture wells per year. Cultures fixed with trichloroacetic acid were stained for 30 minutes with 0.4% (wt/vol) sul-forhodamine B (SRB) dissolved in 1% acetic acid. Unbound dye was removed by four washes with 1% acetic acid, and protein-bound dye was extracted with 10 mM unbuffered Tris base [tris (hydroxymethyl)aminomethane] for determination of optical density in a computer-interfaced, 96-well microtiter plate reader. The SRB assay results were linear with the number of cells and with values for cellular protein measured by both the Lowry and Bradford assays at densities ranging from sparse subconfluence to multilayered supraconfluence. The signal-to-noise ratio at 564 nm was approximately 1.5 with 1,000 cells per well. The sensitivity of the SRB assay compared favorably with sensitivities of several fluorescence assays and was superior to those of both the Lowry and Bradford assays and to those of 20 other visible dyes. The SRB assay provides a colorimetric end point that is nondestructive, indefinitely stable, and visible to the naked eye. It provides a sensitive measure of drug-induced cytotoxicity, is useful in quantitating clonogenicity, and is well suited to high-volume, automated drug screening. SRB fluoresces strongly with laser excitation at 488 nm and can be measured quantitatively at the single-cell level by static fluorescence cytometry.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Involvement of the HER2 pathway in repair of DNA damage produced by chemotherapeutic agents Mol. Cancer Ther., November 1, 2009; 8(11): 3015 - 3023.
|
|
|
|
 |
|
 J. F. Deeken, R. W. Robey, S. Shukla, K. Steadman, A. R. Chakraborty, B. Poonkuzhali, E. G. Schuetz, S. Holbeck, S. V. Ambudkar, and S. E. Bates Identification of Compounds that Correlate with ABCG2 Transporter Function in the National Cancer Institute Anticancer Drug Screen Mol. Pharmacol., November 1, 2009; 76(5): 946 - 956. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.J. Moghaddam, P. Barta, S.G. Mirabolfathinejad, Z. Ammar-Aouchiche, N. T. Garza, T.T. Vo, R. A. Newman, B. B. Aggarwal, C. M. Evans, M. J. Tuvim, et al. Curcumin inhibits COPD-like airway inflammation and lung cancer progression in mice Carcinogenesis, November 1, 2009; 30(11): 1949 - 1956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K.W. To, R. W. Robey, T. Knutsen, Z. Zhan, T. Ried, and S. E. Bates Escape from hsa-miR-519c enables drug-resistant cells to maintain high expression of ABCG2 Mol. Cancer Ther., October 1, 2009; 8(10): 2959 - 2968. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-W. Chien, I-C. Ho, and T.-C. Lee Induction of neoplastic transformation by ectopic expression of human aldo-keto reductase 1C isoforms in NIH3T3 cells Carcinogenesis, October 1, 2009; 30(10): 1813 - 1820. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ali, B. A. Telfer, C. McCrudden, M. O'Rourke, H. D. Thomas, M. Kamjoo, S. Kyle, T. Robson, C. Shaw, D. G. Hirst, et al. Vasoactivity of AG014699, a Clinically Active Small Molecule Inhibitor of Poly(ADP-ribose) Polymerase: a Contributory Factor to Chemopotentiation In vivo? Clin. Cancer Res., October 1, 2009; 15(19): 6106 - 6112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Li, Z.-H. Miao, Z. Chen, Q. Chen, M. Gui, L.-P. Lin, P. Sun, Y.-H. Yi, and J. Ding Echinoside A, a new marine-derived anticancer saponin, targets topoisomerase2{alpha} by unique interference with its DNA binding and catalytic cycle Ann. Onc., September 22, 2009; (2009) mdp335v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Bax, N. Gaspar, S. E. Little, L. Marshall, L. Perryman, M. Regairaz, M. Viana-Pereira, R. Vuononvirta, S. Y. Sharp, J. S. Reis-Filho, et al. EGFRvIII Deletion Mutations in Pediatric High-Grade Glioma and Response to Targeted Therapy in Pediatric Glioma Cell Lines Clin. Cancer Res., September 15, 2009; 15(18): 5753 - 5761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. IZYKOWSKA, E. GEBAROWSKA, M. CEGIELSKI, M. PODHORSKA-OKOLOW, A. PIOTROWSKA, M. ZABEL, and P. DZIEGIEL Effect of Melatonin on Melanoma Cells Subjected to UVA and UVB Radiation in In Vitro Studies In Vivo, September 1, 2009; 23(5): 733 - 738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. IZYKOWSKA, M. CEGIELSKI, E. GEBAROWSKA, M. PODHORSKA-OKOLOW, A. PIOTROWSKA, M. ZABEL, and P. DZIEGIEL Effect of Melatonin on Human Keratinocytes and Fibroblasts Subjected to UVA and UVB Radiation In Vitro In Vivo, September 1, 2009; 23(5): 739 - 745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Gundimeda, J. E. Schiffman, S. N. Gottlieb, B. I. Roth, and R. Gopalakrishna Negation of the cancer-preventive actions of selenium by over-expression of protein kinase C{varepsilon} and selenoprotein thioredoxin reductase Carcinogenesis, September 1, 2009; 30(9): 1553 - 1561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ali, D. A. Heathcote, S. H.B. Kroll, A. S. Jogalekar, B. Scheiper, H. Patel, J. Brackow, A. Siwicka, M. J. Fuchter, M. Periyasamy, et al. The Development of a Selective Cyclin-Dependent Kinase Inhibitor That Shows Antitumor Activity Cancer Res., August 1, 2009; 69(15): 6208 - 6215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Panyosak, J. Manosroi, Y. Rojanasakul, and A. Manosroi Safety assessment of azelaic acid and its derivatives entrapped in nanovesicles Human and Experimental Toxicology, June 1, 2009; 28(6-7): 387 - 392. [Abstract] [PDF]
|
|
|
|
 |
|
 K. SZCZAURSKA-NOWAK, K. DABROWSKA, M. CELKA, A. KURZEPA, D. NEVOZHAY, J. WIETRZYK, K. SWITALA-JELEN, D. SYPER, G. POZNIAK, A. OPOLSKI, et al. Antitumor Effect of Combined Treatment of Mice with Cytostatic Agents and Bacteriophage T4 Anticancer Res, June 1, 2009; 29(6): 2361 - 2370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.R.M. R. Amin, F. R. Khuri, Z. Chen, and D. M. Shin Synergistic Growth Inhibition of Squamous Cell Carcinoma of the Head and Neck by Erlotinib and Epigallocatechin-3-Gallate: The Role of p53-Dependent Inhibition of Nuclear Factor-{kappa}B Cancer Prevention Research, June 1, 2009; 2(6): 538 - 545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-J. Lin, R. Sukarieh, and J. Pelletier Silibinin inhibits translation initiation: implications for anticancer therapy Mol. Cancer Ther., June 1, 2009; 8(6): 1606 - 1612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Gaspar, S. Y. Sharp, S. Pacey, C. Jones, M. Walton, G. Vassal, S. Eccles, A. Pearson, and P. Workman Acquired Resistance to 17-Allylamino-17-Demethoxygeldanamycin (17-AAG, Tanespimycin) in Glioblastoma Cells Cancer Res., March 1, 2009; 69(5): 1966 - 1975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kasneci, N. M. Kemeny-Suss, S. V. Komarova, and L. E. Chalifour Egr-1 negatively regulates calsequestrin expression and calcium dynamics in ventricular cells Cardiovasc Res, March 1, 2009; 81(4): 695 - 702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. H.L. Hamelers, R. W.H.M. Staffhorst, J. Voortman, B. de Kruijff, J. Reedijk, P. M.P. van Bergen en Henegouwen, and A. I.P.M. de Kroon High Cytotoxicity of Cisplatin Nanocapsules in Ovarian Carcinoma Cells Depends on Uptake by Caveolae-Mediated Endocytosis Clin. Cancer Res., February 15, 2009; 15(4): 1259 - 1268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Sahu and S. K. Srivastava The Role of STAT-3 in the Induction of Apoptosis in Pancreatic Cancer Cells by Benzyl Isothiocyanate J Natl Cancer Inst, February 4, 2009; 101(3): 176 - 193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Shukla, R. W. Robey, S. E. Bates, and S. V. Ambudkar Sunitinib (Sutent, SU11248), a Small-Molecule Receptor Tyrosine Kinase Inhibitor, Blocks Function of the ATP-Binding Cassette (ABC) Transporters P-Glycoprotein (ABCB1) and ABCG2 Drug Metab. Dispos., February 1, 2009; 37(2): 359 - 365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ram, O. Uziel, O. Eldan, E. Fenig, E. Beery, S. Lichtenberg, Y. Nordenberg, and M. Lahav Ionizing Radiation Up-regulates Telomerase Activity in Cancer Cell Lines by Post-translational Mechanism via Ras/Phosphatidylinositol 3-Kinase/Akt Pathway Clin. Cancer Res., February 1, 2009; 15(3): 914 - 923. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Milano, Y. Piao, T. LaFortune, and J. de Groot Dasatinib-induced autophagy is enhanced in combination with temozolomide in glioma Mol. Cancer Ther., February 1, 2009; 8(2): 394 - 406. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Gundimeda, J. E. Schiffman, D. Chhabra, J. Wong, A. Wu, and R. Gopalakrishna Locally Generated Methylseleninic Acid Induces Specific Inactivation of Protein Kinase C Isoenzymes: RELEVANCE TO SELENIUM-INDUCED APOPTOSIS IN PROSTATE CANCER CELLS J. Biol. Chem., December 12, 2008; 283(50): 34519 - 34531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Dell'Ovo, E. Bandi, T. Coslovich, C. Florio, M. Sciancalepore, G. Decorti, S. Sosa, P. Lorenzon, T. Yasumoto, and A. Tubaro In Vitro Effects of Yessotoxin on a Primary Culture of Rat Cardiomyocytes Toxicol. Sci., December 1, 2008; 106(2): 392 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Cianchi, L. Papucci, N. Schiavone, M. Lulli, L. Magnelli, M. C. Vinci, L. Messerini, C. Manera, E. Ronconi, P. Romagnani, et al. Cannabinoid Receptor Activation Induces Apoptosis through Tumor Necrosis Factor {alpha}-Mediated Ceramide De novo Synthesis in Colon Cancer Cells Clin. Cancer Res., December 1, 2008; 14(23): 7691 - 7700. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Salhia, N. L. Tran, A. Chan, A. Wolf, M. Nakada, F. Rutka, M. Ennis, W. S. McDonough, M. E. Berens, M. Symons, et al. The Guanine Nucleotide Exchange Factors Trio, Ect2, and Vav3 Mediate the Invasive Behavior of Glioblastoma Am. J. Pathol., December 1, 2008; 173(6): 1828 - 1838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Risinger, E. M. Jackson, L. A. Polin, G. L. Helms, D. A. LeBoeuf, P. A. Joe, E. Hopper-Borge, R. F. Luduena, G. D. Kruh, and S. L. Mooberry The Taccalonolides: Microtubule Stabilizers That Circumvent Clinically Relevant Taxane Resistance Mechanisms Cancer Res., November 1, 2008; 68(21): 8881 - 8888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. White, M. Coetsee, K. Morgan, C. A. Flanagan, R. P. Millar, and Z.-L. Lu A Crucial Role for G{alpha}q/11, But Not G{alpha}i/o or G{alpha}s, in Gonadotropin-Releasing Hormone Receptor-Mediated Cell Growth Inhibition Mol. Endocrinol., November 1, 2008; 22(11): 2520 - 2530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Libertini, I. Iacuzzo, G. Perruolo, S. Scala, C. Ierano, R. Franco, G. Hallden, and G. Portella Bevacizumab Increases Viral Distribution in Human Anaplastic Thyroid Carcinoma Xenografts and Enhances the Effects of E1A-Defective Adenovirus dl922-947 Clin. Cancer Res., October 15, 2008; 14(20): 6505 - 6514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Peklak-Scott, P. K. Smitherman, A. J. Townsend, and C. S. Morrow Role of glutathione S-transferase P1-1 in the cellular detoxification of cisplatin Mol. Cancer Ther., October 1, 2008; 7(10): 3247 - 3255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Dewaele, B. Wasag, J. Cools, R. Sciot, H. Prenen, P. Vandenberghe, A. Wozniak, P. Schoffski, P. Marynen, and M. Debiec-Rychter Activity of Dasatinib, a Dual SRC/ABL Kinase Inhibitor, and IPI-504, a Heat Shock Protein 90 Inhibitor, against Gastrointestinal Stromal Tumor-Associated PDGFRAD842V Mutation Clin. Cancer Res., September 15, 2008; 14(18): 5749 - 5758. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Ehnert, A. K. Nussler, A. Lehmann, and S. Dooley Blood Monocyte-Derived Neohepatocytes as in Vitro Test System for Drug Metabolism Drug Metab. Dispos., September 1, 2008; 36(9): 1922 - 1929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Dubi, L. Gheber, D. Fishman, I. Sekler, and M. Hershfinkel Extracellular zinc and zinc-citrate, acting through a putative zinc-sensing receptor, regulate growth and survival of prostate cancer cells Carcinogenesis, September 1, 2008; 29(9): 1692 - 1700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Breier, N. M. Radio, W. R. Mundy, and T. J. Shafer Development of a High-Throughput Screening Assay for Chemical Effects on Proliferation and Viability of Immortalized Human Neural Progenitor Cells Toxicol. Sci., September 1, 2008; 105(1): 119 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Cheng, R. Samimi, G. Xie, J. Shant, C. Drachenberg, M. Wade, R. J. Davis, G. Nomikos, and J.-P. Raufman Acetylcholine release by human colon cancer cells mediates autocrine stimulation of cell proliferation Am J Physiol Gastrointest Liver Physiol, September 1, 2008; 295(3): G591 - G597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhao, A. Qiu, E. Tsai, M. Jansen, M. H. Akabas, and I. D. Goldman The Proton-Coupled Folate Transporter: Impact on Pemetrexed Transport and on Antifolates Activities Compared with the Reduced Folate Carrier Mol. Pharmacol., September 1, 2008; 74(3): 854 - 862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Kasinski, Y. Du, S. L. Thomas, J. Zhao, S.-Y. Sun, F. R. Khuri, C.-Y. Wang, M. Shoji, A. Sun, J. P. Snyder, et al. Inhibition of I{kappa}B Kinase-Nuclear Factor-{kappa}B Signaling Pathway by 3,5-Bis(2-flurobenzylidene)piperidin-4-one (EF24), a Novel Monoketone Analog of Curcumin Mol. Pharmacol., September 1, 2008; 74(3): 654 - 661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. de Wolf, R. Jansen, H. Yamaguchi, M. de Haas, K. van de Wetering, J. Wijnholds, J. Beijnen, and P. Borst Contribution of the drug transporter ABCG2 (breast cancer resistance protein) to resistance against anticancer nucleosides Mol. Cancer Ther., September 1, 2008; 7(9): 3092 - 3102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Dougan, A. Habtemariam, S. E. McHale, S. Parsons, and P. J. Sadler Catalytic organometallic anticancer complexes PNAS, August 19, 2008; 105(33): 11628 - 11633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mohan and D. Panda Kinetic Stabilization of Microtubule Dynamics by Estramustine Is Associated with Tubulin Acetylation, Spindle Abnormalities, and Mitotic Arrest Cancer Res., August 1, 2008; 68(15): 6181 - 6189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Morgan, A. J. Stewart, N. Miller, P. Mullen, M. Muir, M. Dodds, F. Medda, D. Harrison, S. Langdon, and R. P. Millar Gonadotropin-Releasing Hormone Receptor Levels and Cell Context Affect Tumor Cell Responses to Agonist In vitro and In vivo Cancer Res., August 1, 2008; 68(15): 6331 - 6340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Veltkamp, D. Pluim, M. A.J. van Eijndhoven, M. J. Bolijn, F. H.G. Ong, R. Govindarajan, J. D. Unadkat, J. H. Beijnen, and J. H.M. Schellens New insights into the pharmacology and cytotoxicity of gemcitabine and 2',2'-difluorodeoxyuridine Mol. Cancer Ther., August 1, 2008; 7(8): 2415 - 2425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kano, N. Miyajima, S. Fukuda, and S. Hatakeyama Tripartite Motif Protein 32 Facilitates Cell Growth and Migration via Degradation of Abl-Interactor 2 Cancer Res., July 15, 2008; 68(14): 5572 - 5580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Rizzo, H. Miao, G. D'Souza, C. Osipo, J. Yun, H. Zhao, J. Mascarenhas, D. Wyatt, G. Antico, L. Hao, et al. Cross-talk between Notch and the Estrogen Receptor in Breast Cancer Suggests Novel Therapeutic Approaches Cancer Res., July 1, 2008; 68(13): 5226 - 5235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-Y. Wang, Y. Yin, H. Yuan, T. Sakamaki, H. Okano, and R. I. Glazer Musashi1 Modulates Mammary Progenitor Cell Expansion through Proliferin-Mediated Activation of the Wnt and Notch Pathways Mol. Cell. Biol., June 1, 2008; 28(11): 3589 - 3599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Armeanu, M. Krusch, K. M. Baltz, T. S. Weiss, I. Smirnow, A. Steinle, U. M. Lauer, M. Bitzer, and H. R. Salih Direct and Natural Killer Cell-Mediated Antitumor Effects of Low-Dose Bortezomib in Hepatocellular Carcinoma Clin. Cancer Res., June 1, 2008; 14(11): 3520 - 3528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Sibhatu, P. K. Smitherman, A. J. Townsend, and C. S. Morrow Expression of MRP1 and GSTP1-1 modulate the acute cellular response to treatment with the chemopreventive isothiocyanate, sulforaphane Carcinogenesis, April 1, 2008; 29(4): 807 - 815. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Pariwat, S. Homvisasevongsa, C. Muanprasat, and V. Chatsudthipong A Natural Plant-Derived Dihydroisosteviol Prevents Cholera Toxin-Induced Intestinal Fluid Secretion J. Pharmacol. Exp. Ther., February 1, 2008; 324(2): 798 - 805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Filomeni, P. Turella, M. L. Dupuis, O. Forini, M. R. Ciriolo, M. Cianfriglia, S. Pezzola, G. Federici, and A. M. Caccuri 6-(7-Nitro-2,1,3-benzoxadiazol-4-ylthio)hexanol, a specific glutathione S-transferase inhibitor, overcomes the multidrug resistance (MDR)-associated protein 1-mediated MDR in small cell lung cancer Mol. Cancer Ther., February 1, 2008; 7(2): 371 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wang, P. Guo, X. Wang, Q. Zhou, and J. M. Gallo Preclinical pharmacokinetic/pharmacodynamic models of gefitinib and the design of equivalent dosing regimens in EGFR wild-type and mutant tumor models Mol. Cancer Ther., February 1, 2008; 7(2): 407 - 417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Chan, R. Paulmurugan, O. S. Gheysens, J. Kim, G. Chiosis, and S. S. Gambhir Molecular Imaging of the Efficacy of Heat Shock Protein 90 Inhibitors in Living Subjects Cancer Res., January 1, 2008; 68(1): 216 - 226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. Atcha, A. Syed, B. Wu, N. P. Hoverter, N. N. Yokoyama, J.-H. T. Ting, J. E. Munguia, H. J. Mangalam, J. L. Marsh, and M. L. Waterman A Unique DNA Binding Domain Converts T-Cell Factors into Strong Wnt Effectors Mol. Cell. Biol., December 1, 2007; 27(23): 8352 - 8363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-E. Legrier, C.-P. H. Yang, H.-G. Yan, L. Lopez-Barcons, S. M. Keller, R. Perez-Soler, S. B. Horwitz, and H. M. McDaid Targeting Protein Translation in Human Non Small Cell Lung Cancer via Combined MEK and Mammalian Target of Rapamycin Suppression Cancer Res., December 1, 2007; 67(23): 11300 - 11308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takagi, T. S. Dexheimer, C. Redon, O. Sordet, K. Agama, G. Lavielle, A. Pierre, S. E. Bates, and Y. Pommier Novel E-ring camptothecin keto analogues (S38809 and S39625) are stable, potent, and selective topoisomerase I inhibitors without being substrates of drug efflux transporters Mol. Cancer Ther., December 1, 2007; 6(12): 3229 - 3238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Larson, J. Li, D. H. Chung, and B. M. Evers Molecular mechanisms contributing to glutamine-mediated intestinal cell survival Am J Physiol Gastrointest Liver Physiol, December 1, 2007; 293(6): G1262 - G1271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. O von Bueren, R. Ma, M. Schlumpf, and W. Lichtensteiger Salbutamol exhibits androgenic activity in vitro Br. J. Sports Med., December 1, 2007; 41(12): 874 - 878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. T. Jun, J. Sun, K. Rex, R. Radinsky, R. Kendall, A. Coxon, and T. L. Burgess AMG 102, A Fully Human Anti-Hepatocyte Growth Factor/Scatter Factor Neutralizing Antibody, Enhances the Efficacy of Temozolomide or Docetaxel in U-87 MG Cells and Xenografts Clin. Cancer Res., November 15, 2007; 13(22): 6735 - 6742. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Libertini, I. Iacuzzo, A. Ferraro, M. Vitale, M. Bifulco, A. Fusco, and G. Portella Lovastatin Enhances the Replication of the Oncolytic Adenovirus dl1520 and Its Antineoplastic Activity against Anaplastic Thyroid Carcinoma Cells Endocrinology, November 1, 2007; 148(11): 5186 - 5194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Cho, P. D. Cole, K.-J. Sohn, G. Gaisano, R. Croxford, B. A. Kamen, and Y.-I. Kim Effects of folate and folylpolyglutamyl synthase modulation on chemosensitivity of breast cancer cells Mol. Cancer Ther., November 1, 2007; 6(11): 2909 - 2920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kiakos, A. Sato, T. Asao, P. J. McHugh, M. Lee, and J. A. Hartley DNA sequence selective adenine alkylation, mechanism of adduct repair, and in vivo antitumor activity of the novel achiral seco-amino-cyclopropylbenz[e]indolone analogue of duocarmycin AS-I-145 Mol. Cancer Ther., October 1, 2007; 6(10): 2708 - 2718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kalakonda, S. C. Nallar, P. Gong, D. J. Lindner, S. E. Goldblum, S. P. Reddy, and D. V. Kalvakolanu Tumor Suppressive Protein Gene Associated with Retinoid-Interferon-Induced Mortality (GRIM)-19 Inhibits src-Induced Oncogenic Transformation at Multiple Levels Am. J. Pathol., October 1, 2007; 171(4): 1352 - 1368. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. A. Gant, M. W. D. Wren, M. S. M. Rollins, A. Jeanes, S. S. Hickok, and T. J. Hall Three novel highly charged copper-based biocides: safety and efficacy against healthcare-associated organisms J. Antimicrob. Chemother., August 1, 2007; 60(2): 294 - 299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Pappa, J. Strathmann, M. Lowinger, H. Bartsch, and C. Gerhauser Quantitative combination effects between sulforaphane and 3,3'-diindolylmethane on proliferation of human colon cancer cells in vitro Carcinogenesis, July 1, 2007; 28(7): 1471 - 1477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Mooberry, K. N. Weiderhold, S. Dakshanamurthy, E. Hamel, E. J. Banner, A. Kharlamova, J. Hempel, J. T. Gupton, and M. L. Brown Identification and Characterization of a New Tubulin-Binding Tetrasubstituted Brominated Pyrrole Mol. Pharmacol., July 1, 2007; 72(1): 132 - 140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chattopadhyay, R. Tamari, S. H. Min, R. Zhao, E. Tsai, and I. D. Goldman Commentary: A Case for Minimizing Folate Supplementation in Clinical Regimens with Pemetrexed Based on the Marked Sensitivity of the Drug to Folate Availability Oncologist, July 1, 2007; 12(7): 808 - 815. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-y. Chuang, A. Valster, S. J. Coniglio, J. M. Backer, and M. Symons The atypical Rho family GTPase Wrch-1 regulates focal adhesion formation and cell migration J. Cell Sci., June 1, 2007; 120(11): 1927 - 1934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Brennan, J. A. Sharp, C. Lefevre, D. Topcic, A. Auguste, M. Digby, and K. R. Nicholas The Tammar Wallaby and Fur Seal: Models to Examine Local Control of Lactation J Dairy Sci, June 1, 2007; 90(13_suppl): E66 - E75. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. H. Morrison, J. A. Bauer, J. A. Lupica, Z. Tang, H. Schmidt, J. A. DiDonato, and D. J. Lindner Effect of Inositol Hexakisphosphate Kinase 2 on Transforming Growth Factor beta-activated Kinase 1 and NF-{kappa}B Activation J. Biol. Chem., May 25, 2007; 282(21): 15349 - 15356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Choe, Z. Chen, C. M. Klass, X. Zhang, and D. M. Shin Enhancement of Docetaxel-Induced Cytotoxicity by Blocking Epidermal Growth Factor Receptor and Cyclooxygenase-2 Pathways in Squamous Cell Carcinoma of the Head and Neck Clin. Cancer Res., May 15, 2007; 13(10): 3015 - 3023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Farnie, R. B. Clarke, K. Spence, N. Pinnock, K. Brennan, N. G. Anderson, and N. J. Bundred Novel Cell Culture Technique for Primary Ductal Carcinoma In Situ: Role of Notch and Epidermal Growth Factor Receptor Signaling Pathways J Natl Cancer Inst, April 18, 2007; 99(8): 616 - 627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Aneja, A. M. Ghaleb, J. Zhou, V. W. Yang, and H. C. Joshi p53 and p21 Determine the Sensitivity of Noscapine-Induced Apoptosis in Colon Cancer Cells Cancer Res., April 15, 2007; 67(8): 3862 - 3870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Schafer-Hales, J. Iaconelli, J. P. Snyder, A. Prussia, J. H. Nettles, A. El-Naggar, F. R. Khuri, P. Giannakakou, and A. I. Marcus Farnesyl transferase inhibitors impair chromosomal maintenance in cell lines and human tumors by compromising CENP-E and CENP-F function Mol. Cancer Ther., April 1, 2007; 6(4): 1317 - 1328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. T. Fok, K.-C. Huang, P. C. Holland, and J. Nalbantoglu The Coxsackie and Adenovirus Receptor Binds Microtubules and Plays a Role in Cell Migration J. Biol. Chem., March 9, 2007; 282(10): 7512 - 7521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Frgala, O. Kalous, R. T. Proffitt, and C. P. Reynolds A fluorescence microplate cytotoxicity assay with a 4-log dynamic range that identifies synergistic drug combinations Mol. Cancer Ther., March 1, 2007; 6(3): 886 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Thomas, C. R. Calabrese, M. A. Batey, S. Canan, Z. Hostomsky, S. Kyle, K. A. Maegley, D. R. Newell, D. Skalitzky, L.-Z. Wang, et al. Preclinical selection of a novel poly(ADP-ribose) polymerase inhibitor for clinical trial Mol. Cancer Ther., March 1, 2007; 6(3): 945 - 956. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Shureiqi, X. Zuo, R. Broaddus, Y. Wu, B. Guan, J. S. Morris, and S. M. Lippman The transcription factor GATA-6 is overexpressed in vivo and contributes to silencing 15-LOX-1 in vitro in human colon cancer FASEB J, March 1, 2007; 21(3): 743 - 753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hardcastle, P. Tomlin, C. Norris, J. Richards, M. Cordwell, K. Boxall, M. Rowlands, K. Jones, I. Collins, E. McDonald, et al. A duplexed phenotypic screen for the simultaneous detection of inhibitors of the molecular chaperone heat shock protein 90 and modulators of cellular acetylation Mol. Cancer Ther., March 1, 2007; 6(3): 1112 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Tamvakopoulos, K. Dimas, Z. D. Sofianos, S. Hatziantoniou, Z. Han, Z.-L. Liu, J. H. Wyche, and P. Pantazis Metabolism and Anticancer Activity of the Curcumin Analogue, Dimethoxycurcumin Clin. Cancer Res., February 15, 2007; 13(4): 1269 - 1277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Huang, H. Gao, Y. Chen, H. Zhu, Y. Cai, X. Zhang, Z. Miao, H. Jiang, J. Zhang, H. Shen, et al. Chimmitecan, a Novel 9-Substituted Camptothecin, with Improved Anticancer Pharmacologic Profiles In vitro and In vivo Clin. Cancer Res., February 15, 2007; 13(4): 1298 - 1307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhu, M. Huang, F. Yang, Y. Chen, Z.-H. Miao, X.-H. Qian, Y.-F. Xu, Y.-X. Qin, H.-B. Luo, X. Shen, et al. R16, a novel amonafide analogue, induces apoptosis and G2-M arrest via poisoning topoisomerase II Mol. Cancer Ther., February 1, 2007; 6(2): 484 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Monks, S. Liu, Y. Xu, H. Yu, A. S. Bendelow, and J. A. Moscow Potent cytotoxicity of the phosphatase inhibitor microcystin LR and microcystin analogues in OATP1B1- and OATP1B3-expressing HeLa cells Mol. Cancer Ther., February 1, 2007; 6(2): 587 - 598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Qiu, J. Domarkas, R. Banerjee, N. Merayo, F. Brahimi, J. P. McNamee, B. F. Gibbs, and B. J. Jean-Claude The Combi-Targeting Concept: In vitro and In vivo Fragmentation of a Stable Combi-Nitrosourea Engineered to Interact with the Epidermal Growth Factor Receptor while Remaining DNA Reactive Clin. Cancer Res., January 1, 2007; 13(1): 331 - 340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Neto Cranberry and Its Phytochemicals: A Review of In Vitro Anticancer Studies J. Nutr., January 1, 2007; 137(1): 186S - 193S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Kilker and M. D. Planas-Silva Cyclin D1 Is Necessary for Tamoxifen-Induced Cell Cycle Progression in Human Breast Cancer Cells Cancer Res., December 1, 2006; 66(23): 11478 - 11484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Challacombe, A. Suhrbier, P. G. Parsons, B. Jones, P. Hampson, D. Kavanagh, G. E. Rainger, M. Morris, J. M. Lord, T. T. T. Le, et al. Neutrophils Are a Key Component of the Antitumor Efficacy of Topical Chemotherapy with Ingenol-3-Angelate J. Immunol., December 1, 2006; 177(11): 8123 - 8132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. W. To, Z. Zhan, and S. E. Bates Aberrant Promoter Methylation of the ABCG2 Gene in Renal Carcinoma Mol. Cell. Biol., November 15, 2006; 26(22): 8572 - 8585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Lin, Y.-w. Huang, X.-D. Zhou, and Y. Ma Toxicity of Cerium Oxide Nanoparticles in Human Lung Cancer Cells International Journal of Toxicology, November 1, 2006; 25(6): 451 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. W. Maines, K. J. French, E. B. Wolpert, D. A. Antonetti, and C. D. Smith Pharmacologic Manipulation of Sphingosine Kinase in Retinal Endothelial Cells: Implications for Angiogenic Ocular Diseases Invest. Ophthalmol. Vis. Sci., November 1, 2006; 47(11): 5022 - 5031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ishida, J. C. P. de Mello, D. A. G. Cortez, B. P. D. Filho, T. Ueda-Nakamura, and C. V. Nakamura Influence of tannins from Stryphnodendron adstringens on growth and virulence factors of Candida albicans J. Antimicrob. Chemother., November 1, 2006; 58(5): 942 - 949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-J. Cozzi, P. G. Parsons, S. M. Ogbourne, J. Pedley, and G. M. Boyle Induction of Senescence in Diterpene Ester-Treated Melanoma Cells via Protein Kinase C-Dependent Hyperactivation of the Mitogen-Activated Protein Kinase Pathway. Cancer Res., October 15, 2006; 66(20): 10083 - 10091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. Schroeder, H. Kadara, D. Lotan, J. K. Woo, H.-Y. Lee, W. K. Hong, and R. Lotan Involvement of Mitochondrial and Akt Signaling Pathways in Augmented Apoptosis Induced by a Combination of Low Doses of Celecoxib and N-(4-Hydroxyphenyl) Retinamide in Premalignant Human Bronchial Epithelial Cells Cancer Res., October 1, 2006; 66(19): 9762 - 9770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Azrak, C. L. Frank, X. Ling, H. K. Slocum, F. Li, B. A. Foster, and Y. M. Rustum The mechanism of methylselenocysteine and docetaxel synergistic activity in prostate cancer cells. Mol. Cancer Ther., October 1, 2006; 5(10): 2540 - 2548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chattopadhyay, R. Zhao, E. Tsai, V. L. Schramm, and I. D. Goldman The effect of a novel transition state inhibitor of methylthioadenosine phosphorylase on pemetrexed activity. Mol. Cancer Ther., October 1, 2006; 5(10): 2549 - 2555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Karst, D. L. Dai, J. Q. Cheng, and G. Li Role of p53 Up-regulated Modulator of Apoptosis and Phosphorylated Akt in Melanoma Cell Growth, Apoptosis, and Patient Survival. Cancer Res., September 15, 2006; 66(18): 9221 - 9226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liang, R. A Brekken, and S. M Hyder Vascular endothelial growth factor induces proliferation of breast cancer cells and inhibits the anti-proliferative activity of anti-hormones. Endocr. Relat. Cancer, September 1, 2006; 13(3): 905 - 919. [Abstract] [Full Text] [PDF]
|
|