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JNCI Journal of the National Cancer Institute 1990 82(11):927-933; doi:10.1093/jnci/82.11.927
© 1990 by Oxford University Press
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Journal of the National Cancer Institute, Vol. 82, No. 11, 927-933, June 6, 1990
© 1990 Oxford University Press

Determination of Benzo[a]pyrene Diol Epoxide—DNA Adducts in White Blood Cell DNA From Coke-Oven Workers: The Impact of Smoking

Frederik Jan van Schooten, Flora E. van Leeuwen, Michel J. X. Hillebrand, Marieke E. de Rijke, Augustinus A. M. Hart, Henk G. van Veen, Samuel Oosterink, Erik Kriek*

Division of Chemical Carcinogenesis, The Netherlands Cancer Institute Amsterdam, The Netherlands.
Division of Clinical Oncology, The Netherlands Cancer Insitute
Hoogoven Groep V.B. IJmuiden, The Netherlands.

*Correspondence to: Erik Kriek, Ph.D., Division of Chemical Carcinogenesis, The Netherlands Cancer Institute (Antoni van Leeuenhoekhuis), 121 Plesmanlann, 1066 CX Amsterdam, The Netherlands.

We have undertaken a study among coke-oven workers to test the feasibility of an enzyme-linked immunosorbent assay with anti-trans-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahy-dro-benzo[a]pyrene-DNA antibodies for monitoring occupational exposure to poly cyclic aromatic hydrocarbons (PAH). Coke-oven workers are occupationally exposed to relatively high levels of PAH and are at increased risk for lung cancer. Three blood samples were collected from each of the 56 coke-oven workers exposed to PAH and 44 unexposed workers employed in a steel-rolling factory of the same plant. In addition, PAH levels were measured in ambient air by personal sampling, and the excretion of 1-hydroxypyrene in urine was also measured on 3 consecutive working days. All participants were interviewed regarding working conditions, personal hygiene, and smoking habits. The results showed that the coke-oven workers were exposed to substantial concentrations of atmospheric PAH [1–186 µg/m3), including benzo[a]pyrene (0.1–7.8 µg/m3) and pyrene (0.6–23.6 µg/m3). Both benzo[a]pyrene and pyrene were shown to be representative for the whole group of PAH. Forty-seven percent of the coke-oven workers had detectable levels of PAH–DNA adducts in their white blood cells, compared with 30% of the controls. In both groups, smokers had significantly higher levels of PAH-DNA adducts than did nonsmokers. At one site, we found the correlation positive between DNA adducts and the duration of exposure (r= .47, p= .005). Generally, the correlation was not significant between PAH–DNA adducts in blood and the concentration of PAH in the air and 1-hydroxypyrene in urine. [J Natl Cancer Inst 82: 927–933, 1990]


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