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JNCI Journal of the National Cancer Institute 1988 80(16):1333-1337; doi:10.1093/jnci/80.16.1333
© 1988 by Oxford University Press
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Journal of the National Cancer Institute, Vol. 80, No. 16, 1333-1337, October 19, 1988
© 1988 Oxford University Press

Improved Procedure for Determination of Serum Lipid-Associated Sialic Acid: Application for Early Diagnosis of Colorectal Cancer2

Carmen Tautu3, Gary Verazin4, Joseph J. Prorok3,4, Jack A. Alhadeff3,4,5,*

3Department of Chemistry, Lehigh University Bethlehem, PA
4Department of Surgery, Lehigh Valley Hospital Center Allentown, PA

*Correspondence to: Jack A. Alhadeff, Ph.D., Department of Chemistry, Mudd Bldg., No. 6, Lehigh University, Bethlehem, PA 18015.

We have developed a simple and reliable procedure for determining serum levels of lipid-associated sialic acid (LASA) in crude preparations. This method extracts essentially all gan-gliosides, excludes glycoprotein-bound sialic acuid, and gives LASA values (0.5–1.0 mg/dL) in good agreement with values for isolated serum gangliosides. The procedure was used to determine serum levels of LASA in patients with colorectal cancer, in patients with non-malignant diseases (pathological control subjects), and in normal control subjects), The results indicated that the percentages of total sialic acid (TSA) comprised by LASA (LASA/TSA x 100) were elevated in patients with the earliest stages of colorectal cancer, compared with percentages in normal control subjects (P<.001) and pathological control subjects (P <.01).


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