Instability of BCR–ABL Gene in Primary and Cultured Chronic Myeloid Leukemia Stem Cells
J. Natl. Cancer Inst. Jiang et al.
99: 680
Supplementary Data
Files in this Data Supplement:
- Supplementary Table 1 -
Allele-specific reverse transcriptase PCR strategy used to screen specific BCR-ABL kinase domain mutations
- Supplementary Fig. 1 -
Allele-specific real-time PCR analysis of a BCR-ABL kinase domain mutation (T315A). The left panel shows the quantitative real time PCR SYBR green fluorescence versus cycle number for analyses of separate 10-fold serial dilutions of T315A mutant plasmid DNA in a plasmid DNA preparation containing the wild type BCR-ABL kinase domain in order to determine the optimal conditions for detecting the T315A mutant. The middle panel shows a melting curve analysis of the specific T315A mutant plasmid corresponding to different dilutions of mutant and wild type BCR-ABL plasmids, as indicated. The right panel shows a linear regression of the relationship between the Ct values (with 95% confidence intervals) and the logarithm of the copy numbers from a series of measurements of independent dilutions of the mutant plasmid DNA.
