Epigenetic Modulation of Tumor Suppressor CCAAT/Enhancer Binding Protein 
Activity in Lung Cancer
J. Natl. Cancer Inst. Tada et al.
98: 396
Supplementary Figures
The following supplementary figures accompany this Article:
Files in this Data Supplement:
- Supplementary Figure 1 -
Summary of primer sequences, polymerase chain reaction (PCR)
conditions, restriction enzymes used for bisulfite-PCR, and PCR primer pairs
- Supplementary Figure 2 -
Summary of primer sequences and polymerase chain reaction (PCR)
conditions for chromatin immunoprecipitation (ChIP)
- Supplementary Figure 3 -
Schematic representation of the CAAT/enhancer-binding protein
(C/EBP)-alpha locus. CpG sites, locations of BssHII restriction sites in the C/EBP-alpha promoter
region, the probes used for Southern and northern blot analysis, the CpG island,
5’untranslated region (UTR), exon 1, and 3’UTR are shown. The polymerase chain
reaction (PCR) primer locations used in the methylation analysis are shown. Short
vertical bars indicate individual CG dinucleotides. Numbers indicate the location relative
to the transcription start site (arrow). COBRA = combined bisulfite restriction analysis.
- Supplementary Figure 4 -
Methylation of the CAAT/enhancer-binding protein (C/EBP)-alpha promoter in primary lung cancer, normal lung tissues and in lung cancer
cell lines. A) Summary of bisulfite genomic sequencing for normal lung tissue and lung
cancer cell lines. Open and closed areas represent unmethylated and methylated CpG
dinucleotides respectively. Positions relative to the transcriptional start site are shown at
the top. Sample number or cell line name is shown on the left. (n) indicates the number of
clones used for sequencing. SssI = in vitro-methylated control; PBL = peripheral blood
lymphocytes. B) Summary of bisulfite genomic sequencing for lung cancer tissues and
adjacent non-cancerous tissues by microdissection. Sample number is given on the left. N = normal; T = tumor.
