Tumor Vascular Permeability, Accumulation, and Penetration of Macromolecular Drug Carriers
J. Natl. Cancer Inst. Dreher et al.
98: 335
Supplemental Movies
The following supplemental movies accompany this Article:
Files in this Data Supplement:
- Supplemental Movie 1
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Three-dimensional tumor vasculature. The tumor vasculature lumen was visualized by perfusion with 2-MDa fluorescein-labeled dextran. The movie rotates 360 degrees about a y-axis and was reconstructed from a tumor tissue specimen with an area of approximately 460 by 460 by 80 μm.
- Supplemental Movie 2
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Distribution of 3.3-kDa dextran. The distribution of 3.3-kDa dextran (fluorescein-labeled dextran, green) and location of the tumor vasculature (rhodamine-labeled 2-MDa dextran, red) was imaged for a 30-minute period at 5-second intervals. The data are shown as a single 7.1-μm optical slice with an area of 460 by 460 μm.
- Supplemental Movie 3
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Distribution of 70-kDa dextran. The distribution of 70-kDa dextran (fluorescein-labeled dextran, green) and location of the tumor vasculature (rhodamine-labeled 2-MDa dextran, red) was imaged for a 30-minute period at 5-second intervals. The data are shown as a single 7.1-μm optical slice with an area of 460 by 460 μm.
- Supplemental Movie 4
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Distribution of 2-MDa dextran. The distribution of 2-MDa dextran (fluorescein-labeled dextran, green) and location of the tumor vasculature (rhodamine-labeled 2-MDa dextran, red) was imaged for a 30-minute period at 5-second intervals. Areas of focal hyperpermeability have more intense green fluorescence and are associated with the wall of the blood vessel that extend slightly into interstitial space. The data are shown as a single 7.1-μm optical slice with an area of 460 by 460 μm.
