YC-1: A Potential Anticancer Drug Targeting Hypoxia-Inducible Factor 1
J. Natl. Cancer Inst.
Yeo et al. 95 (7): 516.
Supplemental Figure
Files in this Data Supplement:
- Figure -
Detection of natural killer cells in the spleen and Hep3B tumors from mice treated with YC-1.
The numbers of natural killer (NK) cells that infiltrated the Hep3B tumors in mice treated with YC-1 (30 μ-g/g) or the vehicle (dimethyl sulfoxide) were determined by immunostaining tumor sections with an anti-asialo GM1 antibody, which is specific for NK cells. Tissue (spleen) and tumor sections were incubated with a rabbit polyclonal anti-asialo GM1 antibody (Wako Chemicals, Chuo-Ku, Osaka, 1:100 dilution in the blocking solution), a species-specific biotinylated secondary antibody, and the avidin-biotin-horseradish peroxidase complex was used to localize the bound antibodies, with diaminobenzidine as the final chromogen. All immunostained sections were lightly counterstained with hematoxylin. Sections of mouse spleen were stained as a positive control. Representative sections are shown. Arrows identify NK cells.
