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The following supplemental figure accompanies the Article by Ito et al., J Natl Cancer Inst 2002;94:1080–91:
Supplemental Figure 1. Effects of inhibition or overexpression
of glucose transporter-1 (Glut1) on matrix metalloproteinase (MMP)-2 mRNA expression in the rhabdomyosarcoma
cell line RD.
mRNA was isolated from RD cells transfected with Glut-1 antisense (lane 1) or sense (lane 2) oligonucleotides, from RD cells transfected with Glut-1 (lane 3) or control (lane 4) cDNA expression vectors, from RD cells treated with transfection reagents (lane 5), or from RD cells (lane 6). Expression of Glut-1 and MMP-2 mRNA was assessed by northern blot analysis. Ethidium bromide-stained 18S and 28S ribosomal RNA is shown as a control to ensure equal loading of lanes.
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