© 2003 by Oxford University Press
Journal of the National Cancer Institute, Vol. 95, No. 3, 237-241,
February 5, 2003
© 2003 Oxford University Press
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Effects of Dietary Selenium Supplementation on DNA Damage and Apoptosis in Canine Prostate
Affiliations of authors: D. J. Waters, S. Shen, D. M. Cooley, Department of Veterinary Clinical Sciences, Purdue University, West Lafayette, IN, and Gerald P. Murphy Cancer Foundation, Seattle, WA; D.G. Bostwick, J. Qian, Bostwick Laboratories, Richmond, VA; G. F. Combs, Jr., Division of Nutritional Sciences, Cornell University, Ithaca, NY; L. T. Glickman (Department of Veterinary Pathobiology), C. Oteham, D. Schlittler (Department of Veterinary Clinical Sciences), Purdue University; J. S. Morris; University of Missouri–Columbia Research Reactor Center, Columbia.
Correspondence to: David J. Waters, D.V.M., Ph.D., Purdue University, School of Veterinary Medicine, 625 Harrison St., West Lafayette, IN 47907–2026 (e-mail: dwaters{at}gpmcf.org).
ABSTRACT
The trace mineral selenium inhibits cancer development in a variety of experimental animal models. We used an in vivo canine model to evaluate the effects of dietary selenium supplementation on DNA damage in prostate tissue and on apoptosis in prostate epithelial cells. Sexually intact elderly male beagle dogs were randomly assigned to receive an unsupplemented diet (control group) or diets that were supplemented with selenium (treatment group), either as selenomethionine or as high-selenium yeast at 3 µg/kg or 6 µg/kg body weight per day for 7 months. The extent of DNA damage in prostate cells and in peripheral blood lymphocytes, as determined by the alkaline comet assay, was lower among the selenium-supplemented dogs than among the control dogs (prostate P<.001; peripheral blood lymphocytes P = .003; analysis of variance) but was not associated with the activity of the antioxidant enzyme glutathione peroxidase in plasma. The median number of terminal deoxynucleotidyl transferase–mediated dUTP nick end-labeling-positive (i.e., apoptotic) prostate epithelial cells was 3.7 (interquartile range = 1.1–7.6) for the selenium-supplemented dogs and 1.7 (interquartile range = 0.2–2.8) for the control dogs ( P = .04, Mann–Whitney U test). These data suggest that dietary selenium supplementation decreases DNA damage and increases epithelial cell apoptosis within the aging canine prostate.
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