© 1999 by Oxford University Press
Journal of the National Cancer Institute, Vol. 91, No. 2, 176-181,
January 20, 1999
© 1999 Oxford University Press
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Influence of Dietary Calcium and Vitamin D on Diet-Induced Epithelial Cell Hyperproliferation in Mice
Affiliations of authors: L. Xue, Memorial Sloan-Kettering Cancer Center and Strang Cancer Prevention Center, New York, NY, and Cell Biology Laboratory, Laboratory Center of Medical Sciences, Henan Medical University, Zhengzhou, China; M. Lipkin, H. Newmark, Memorial Sloan-Kettering Cancer Center and Strang Cancer Prevention Center; J. Wang, Cell Biology Laboratory, Laboratory Center of Medical Sciences, Henan Medical University.
Correspondence to: Martin Lipkin, M.D., Strang Cancer Research Laboratory at The Rockefeller University, 1230 York Ave., New York, NY 10021.
BACKGROUND: Previous epidemiologic and laboratory studies, including some from our own laboratory, have suggested that a high-fat diet increases risk of cancer development in the pancreas, prostate, colon, and breast and that carcinogenesis in some of these organs may be influenced by alterations in dietary calcium and vitamin D. In this study, we sought to investigate the effect of added dietary calcium or vitamin D on the development of epithelial cell hyperproliferation induced by a Western-style diet in the exocrine pancreas, prostate, and mammary gland of mice. METHODS: Four-week-old C57BL/6J mice were given either a control diet (American Institute of Nutrition [AIN]-76A), a Western-style diet (containing reduced calcium and vitamin D and the fat level of the average human Western diet), or a putative chemopreventive diet (a Western-style diet with the addition of dietary calcium and vitamin D). Nine weeks after dietary intervention, osmotic pumps were implanted in the mice to provide 3 days of bromodeoxyuridine (BrdU) infusion. All P values are two-sided. RESULTS: Mice on the Western-style diet had statistically significant increases in BrdU-labeling indices of epithelial cells in the interlobular (P = .015) and intralobular (P = .012) ducts and centroacinar cells (P = .001) of the pancreatic duct system, the dorsal lobe of the prostate (P = .045), and the terminal ducts of the mammary gland (P = .032), compared with mice in the respective control diet groups. Adding dietary calcium and vitamin D markedly suppressed the Western-style diet-induced hyperproliferation of epithelial cells in those tissues (P = .001-.033). CONCLUSIONS: This study confirms previous findings that a Western-style diet produces hyperproliferation of epithelial cells in several organs and that the changes can be prevented by increasing dietary calcium and vitamin D alone.
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