Skip Navigation

JNCI Journal of the National Cancer Institute 1999 91(18):1563-1568; doi:10.1093/jnci/91.18.1563
© 1999 by Oxford University Press
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Jülicher, K.
Right arrow Articles by Schütte, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jülicher, K.
Right arrow Articles by Schütte, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of the National Cancer Institute, Vol. 91, No. 18, 1563-1568, September 15, 1999
© 1999 Oxford University Press

REPORTS

Novel Tumor Suppressor Locus in Human Chromosome Region 3p14.2

Knut Jülicher, Guido Marquitan, Nicola Werner, Walter Bardenheuer, Lydia Vieten, Frank Bröcker, Hüsnü Topal, Siegfried Seeber, Bertram Opalka, Jochen Schütte

Affiliation of authors: Innere Klinik und Poliklinik (Tumorforschung), Universitätsklinikum Essen, Germany.

Correspondence to: Bertram Opalka, Ph.D., Innere Klinik und Poliklinik (Tumorforschung), Universitätsklinikum Essen, Hufelandstr. 55, D-45122 Essen, Germany (e-mail: bertram.opalka{at}uni-essen.de).

or
Correspondence to:Jochen Schütte, M.D., Innere Klinik und Poliklinik (Tumorforschung), Universitätsklinikum Essen, Hufelandstr. 55, D-45122 Essen, Germany (e-mail: jochen.schuette{at}uni-essen.de).

BACKGROUND: Alterations of chromosome region 3p14 are observed in numerous human malignancies. Because the pattern of allelic losses suggests the existence of at least one tumor suppressor gene within this region, we established a library of yeast artificial chromosomes (YACs) containing contiguous human 3p14 sequences to permit a search for tumor suppressor loci within the 3p14 region by use of functional complementation. METHODS: YACs specific for human chromosome region 3p14 were transduced by spheroplast fusion into cells of the human nonpapillary renal carcinoma cell line RCC-1, which shows a cytogenetically detectable 3p deletion and is tumorigenic in nude mice. RESULTS: We identified a 3p14.2-specific YAC clone, located in the vicinity of the fragile histidine triad (FHIT) gene (but toward the telomere), that is capable of inducing sustained suppression of tumorigenicity in nude mice and of activating cellular senescence in vitro. Among 23 mice given injections of RCC-1 cells containing this YAC, 16 (70%) remained tumor free for at least 6 months, whereas tumor formation occurred after a median of 6 weeks in control mice given injections of either RCC-1 parental cells or a revertant cell line (in which the YAC had lost all human sequences) or RCC-1 parental cells containing other, unrelated YACs. Similar results were obtained following microcell-mediated transfer of the entire human chromosome 3. CONCLUSION: These data provide strong evidence for the existence of a novel tumor suppressor locus adjacent to the previously identified candidate tumor suppressor gene, FHIT, in 3p14.2. Positional cloning of the novel suppressor element within the 3p14.2-specific YAC and the sequence's molecular and functional characterization should add to the understanding of the pathogenesis of renal cell carcinoma and other human tumors that exhibit 3p14 aberrations.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
R.-B. Yang, C. K. D. Ng, S. M. Wasserman, L. G. Komuves, M. E. Gerritsen, and J. N. Topper
A Novel Interleukin-17 Receptor-like Protein Identified in Human Umbilical Vein Endothelial Cells Antagonizes Basic Fibroblast Growth Factor-induced Signaling
J. Biol. Chem., August 29, 2003; 278(35): 33232 - 33238.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
D. Bodmer, W. van den Hurk, J. J. M. van Groningen, M. J. Eleveld, G. J. M. Martens, M. A. J. Weterman, and A. Geurts van Kessel
Understanding familial and non-familial renal cell cancer
Hum. Mol. Genet., October 1, 2002; 11(20): 2489 - 2498.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Shiraishi, T. Druck, K. Mimori, J. Flomenberg, L. Berk, H. Alder, W. Miller, K. Huebner, and C. M. Croce
Sequence conservation at human and mouse orthologous common fragile regions, FRA3B/FHIT and Fra14A2/Fhit
PNAS, April 18, 2001; (2001) 91095898.
[Abstract] [Full Text]

Home page
 JNCI J Natl Cancer InstHome page
F. J. Kaye
Closing in on Another Renal Cancer Suppressor Locus Near Chromosome 3p14
J Natl Cancer Inst, September 15, 1999; 91(18): 1528 - 1529.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Shiraishi, T. Druck, K. Mimori, J. Flomenberg, L. Berk, H. Alder, W. Miller, K. Huebner, and C. M. Croce
Sequence conservation at human and mouse orthologous common fragile regions, FRA3B/FHIT and Fra14A2/Fhit
PNAS, May 8, 2001; 98(10): 5722 - 5727.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.