Skip Navigation

JNCI Journal of the National Cancer Institute 1999 91(16):1398-1403; doi:10.1093/jnci/91.16.1398
© 1999 by Oxford University Press
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Tremblay, C.
Right arrow Articles by Sirois, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tremblay, C.
Right arrow Articles by Sirois, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of the National Cancer Institute, Vol. 91, No. 16, 1398-1403, August 18, 1999
© 1999 Oxford University Press

REPORTS

Induction of Prostaglandin G/H Synthase-2 in a Canine Model of Spontaneous Prostatic Adenocarcinoma

Claudine Tremblay, Monique Doré, Philip N. Bochsler, Jean Sirois

Affiliations of authors: C. Tremblay, M. Doré (Département de Pathologie et Microbiologie), J. Sirois (Centre de Recherche en Reproduction Animale), Faculté de Médecine Vétérinaire, Université de Montréal, Québec, Canada; P. N. Bochsler, Department of Pathology, University of Tennessee, Knoxville.

Correspondence to: Jean Sirois, D.V.M., M.Sc., Ph.D., Centre de Recherche en Reproduction Animale, Faculté de Médecine Vétérinaire, Université de Montréal, C.P. 5000, St-Hyacinthe, Québec, Canada J2S 7C6 (e-mail: siroisje{at}medvet.umontreal.ca).

BACKGROUND: Prostate cancer is the most frequently occurring cancer in men in the United States, with an estimated 179 300 new cases in 1999. The induction of prostaglandin G/H synthase (PGHS), a key rate-limiting enzyme in prostaglandin biosynthesis, has been implicated in various cancers, most notably in colorectal cancers; however, the induction of PGHS expression in prostate cancer in vivo has not been reported for any species. The dog is the only nonhuman species that frequently develops spontaneous cancer of the prostate with increasing age, and the objective of this study was to determine whether PGHS isoenzymes were expressed in canine prostatic adenocarcinomas. METHODS: Four normal canine prostatic tissues and 24 canine prostatic adenocarcinomas were studied by means of immunohistochemistry and immunoblot analysis, using polyclonal antibodies specific for each of the two PGHS isoenzymes, PGHS-1 and PGHS-2. All P values were obtained by use of two-sided Fisher's exact tests. RESULTS: PGHS-1 immunostaining was localized to stromal fibroblasts and vascular endothelium in normal and cancerous prostates. PGHS-2 was not detected in normal prostates, but it was expressed by epithelial tumor cells in 18 (75%) of the 24 adenocarcinomas (P = .01). Immunoblot analysis confirmed the presence of PGHS-1 (69 000 molecular weight) in normal and cancerous tissues and the expression of PGHS-2 (72 000- to 74 000-molecular-weight doublet) only in prostatic adenocarcinomas. CONCLUSION: To our knowledge, these results demonstrate for the first time that PGHS-2 is induced in the majority of canine spontaneous prostatic adenocarcinomas and suggest that its expression may be involved in prostate cancer.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
J. Huh, A. Liepins, J. Zielonka, C. Andrekopoulos, B. Kalyanaraman, and A. Sorokin
Cyclooxygenase 2 Rescues LNCaP Prostate Cancer Cells from Sanguinarine-Induced Apoptosis by a Mechanism Involving Inhibition of Nitric Oxide Synthase Activity.
Cancer Res., April 1, 2006; 66(7): 3726 - 3736.
[Abstract] [Full Text] [PDF]

Home page
 Vet PatholHome page
D. A. Heller, C. A. Clifford, M. H. Goldschmidt, D. E. Holt, F. S. Shofer, A. Smith, and K. U. Sorenmo
Cyclooxygenase-2 Expression is Associated with Histologic Tumor Type in Canine Mammary Carcinoma
Vet. Pathol., November 1, 2005; 42(6): 776 - 780.
[Abstract] [Full Text] [PDF]

Home page
 Vet PatholHome page
M. Dore, S. Chevalier, and J. Sirois
Estrogen-dependent Induction of Cyclooxygenase-2 in the Canine Prostate In Vivo
Vet. Pathol., January 1, 2005; 42(1): 100 - 103.
[Abstract] [Full Text] [PDF]

Home page
 Vet PatholHome page
S. L. Beam, K. M. Rassnick, A. S. Moore, and S. P. McDonough
An Immunohistochemical Study of Cyclooxygenase-2 Expression in Various Feline Neoplasms
Vet. Pathol., September 1, 2003; 40(5): 496 - 500.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Liu, W.-Y. Ma, A. M. Bode, Y. Zhang, and Z. Dong
NS-398 and Piroxicam Suppress UVB-induced Activator Protein 1 Activity by Mechanisms Independent of Cyclooxygenase-2
J. Biol. Chem., January 17, 2003; 278(4): 2124 - 2130.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
S. Lajoie, J. Sirois, and M. Dore
Induction of Cyclo-oxygenase-2 Expression in Naturally Occurring Gastric Ulcers
J. Histochem. Cytochem., July 1, 2002; 50(7): 923 - 934.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. Boutemmine, N. Bouchard, D. Boerboom, H. E. Jones, A. K. Goff, M. Dore, and J. Sirois
Molecular Characterization of Canine Prostaglandin G/H Synthase-2 and Regulation in Prostatic Adenocarcinoma Cells in Vitro
Endocrinology, March 1, 2002; 143(3): 1134 - 1143.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
M. J. Thun, S. J. Henley, and C. Patrono
Nonsteroidal Anti-inflammatory Drugs as Anticancer Agents: Mechanistic, Pharmacologic, and Clinical Issues
J Natl Cancer Inst, February 20, 2002; 94(4): 252 - 266.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
E. M. P. de Almeida, C. Piche, J. Sirois, and M. Dore
Expression of Cyclo-oxygenase-2 in Naturally Occurring Squamous Cell Carcinomas in Dogs
J. Histochem. Cytochem., July 1, 2001; 49(7): 867 - 876.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.