© 1998 by Oxford University Press
Journal Of The National Cancer Institute, Vol 90, 684-690, Copyright © 1998 by Oxford University Press
N Yahata, K Ohyashiki, JH Ohyashiki, H Iwama, S Hayashi, K Ando, T Hirano, T Tsuchida, H Kato, JW Shay and K Toyama
BACKGROUND: Telomerase, a ribonucleoprotein enzyme that functions in the
maintenance of telomeres (specialized structures at the ends of
chromosomes), has been reported to be a novel diagnostic marker for
malignant diseases. We sought to determine whether measurement of
telomerase activity in bronchial washings is of value in the diagnosis of
lung cancer. METHODS: Extracts of cells in bronchial washings were analyzed
for telomerase activity by use of a telomeric repeat amplification protocol
(TRAP) assay. Telomerase activity inside cells was evaluated by use of an
in situ TRAP assay. The results of both TRAP assays were compared with
those obtained from cytologic examination, which employed standard
Papanicolaou staining. RESULTS: When results from the two TRAP assays were
combined, telomerase activity was detected in bronchial washings from 18
(82%; 95% confidence interval [CI] = 60%-95%) of 22 patients with lung
cancer. In contrast, cancer cells were detected by cytologic examination in
the bronchial washings of nine (41%; 95% CI = 21%-64%) of the same 22
patients, a statistically significant difference (two-sided P = .0061). In
patients with lung cancer, telomerase-positive cells could be detected in
bronchial washings irrespective of tumor location--11 of 14 (79%; 95% CI =
49%-95%) peripheral cancerous lesions and seven of eight (88%; 95% CI =
47%-100%) central cancerous lesions were detected by use of TRAP assays
(for comparison, two-sided P = .5349). CONCLUSIONS: A high percentage of
patients with lung cancers had detectable telomerase activity in bronchial
washings. Thus, the use of a cell extract-based or an in situ TRAP assay in
addition to cytologic examination may make the diagnosis of lung cancer
more reliable.
ARTICLES
Telomerase activity in lung cancer cells obtained from bronchial washings
First Department of Internal Medicine, Tokyo Medical College, Japan.
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