© 1992 by Oxford University Press
Journal of the National Cancer Institute, Vol. 84, No. 21, 1633-1638,
November 4, 1992
© 1992 Oxford University Press
Role of the SIKVAV Site of Laminin in Promotion of Angiogenesis and Tumor Growth: An In Vivo Matrigel Model
Laboratory of Developmental Biology, National Institute of Dental Research Bethesda, Md.
*Correspondence to:Maura C. Kibbey, Ph.D., National Institutes of Health, Bldg. 30, Rm. 402, Bethesda, MD 20892.
Background: Angiogenesis (vascularization) has a critical role in tumor growth and metastasis, and peptides containing the SIKVAV amino acid sequence (Ser-Ile-Lys-Val-Ala-Val) have been shown to stimulate many angiogenic activities in vitro. The use of model systems to identify agents that stimulate or inhibit angiogenesis may lead to the development of new antitumor strategies. Purpose: Our purpose was to use an in vivo murine model system to study the angiogenic activity of a synthetic peptide derived from the laminin A protein chain and containing the SIKVAV amino acid sequence. We also examined the ability of the peptide to enhance tumor growth in vivo. Methods: The SIKVAV-containing peptide was mixed with Matrigel, a reconstituted basement membrane extract used to assay stimulation of angiogenesis. The mixture was subcutaneously injected into C57BL/6 mice. At various times after injection, the Matrigel plug was excised, and angiogenic activity was assessed by histologic examination and immunohistochemical staining with an antibody to the von Wille-brand factor (vWF), an endothelium-specific antigen. In other experiments, the mixture of peptide and Matrigel was co-injected with B16F10 murine melanoma cells into C57BL/6 mice, and the resultant tumors were assessed for size and vascularization.
Results: When co-injected with Matrigel at doses as low as 10 µg, the SIKVAV-containing peptide stimulated angiogenesis fourfold greater than that seen in controls, and maximum angiogenic activity was observed 2 weeks after injection. This peptide was angiogenic in a dose-dependent manner up to a 100-µg dose. When co-injected with Matrigel and B16F10 melanoma cells, the peptide enhanced tumor growth by approximately 2.5-fold, and tumor vascularization was significantly increased (P =.027) over that observed after injection with melanoma cells and Matrigel alone. Conclusions: These data demonstrate that the laminin-derived SIKVAV-containing peptide is angiogenic in a new in vivo model system and can enhance tumor vascularization and growth. [J Natl Cancer Inst 84: 1633–1638, 1992]
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  V. M. Freitas, V. F. Vilas-Boas, D. C. Pimenta, V. Loureiro, M. A. Juliano, M. R. Carvalho, J. J.V. Pinheiro, A. C.M. Camargo, A. S. Moriscot, M. P. Hoffman, et al. SIKVAV, a Laminin {alpha}1-Derived Peptide, Interacts with Integrins and Increases Protease Activity of a Human Salivary Gland Adenoid Cystic Carcinoma Cell Line through the ERK 1/2 Signaling Pathway Am. J. Pathol., July 1, 2007; 171(1): 124 - 138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-J. Lee, E.-O. Lee, Y.-H. Rhee, K. S. Ahn, G.-X. Li, C. Jiang, J. Lu, and S.-H. Kim An oriental herbal cocktail, ka-mi-kae-kyuk-tang, exerts anti-cancer activities by targeting angiogenesis, apoptosis and metastasis Carcinogenesis, December 1, 2006; 27(12): 2455 - 2463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yasui, H. Yamamoto, C. Y. Ngan, B. Damdinsuren, Y. Sugita, H. Fukunaga, J. Gu, M. Maeda, I. Takemasa, M. Ikeda, et al. Antisense to Cyclin D1 Inhibits Vascular Endothelial Growth Factor-Stimulated Growth of Vascular Endothelial Cells: Implication of Tumor Vascularization Clin. Cancer Res., August 1, 2006; 12(15): 4720 - 4729. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Givant-Horwitz, B. Davidson, and R. Reich Laminin-Induced Signaling in Tumor Cells: The Role of the Mr 67,000 Laminin Receptor Cancer Res., May 15, 2004; 64(10): 3572 - 3579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Hembrough, J. F. Ruiz, B. M. Swerdlow, G. M. Swartz, H. J. Hammers, L. Zhang, S. M. Plum, M. S. Williams, D. K. Strickland, and V. S. Pribluda Identification and characterization of a very low density lipoprotein receptor-binding peptide from tissue factor pathway inhibitor that has antitumor and antiangiogenic activity Blood, May 1, 2004; 103(9): 3374 - 3380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Scapini, M. Morini, C. Tecchio, S. Minghelli, E. Di Carlo, E. Tanghetti, A. Albini, C. Lowell, G. Berton, D. M. Noonan, et al. CXCL1/Macrophage Inflammatory Protein-2-Induced Angiogenesis In Vivo Is Mediated by Neutrophil-Derived Vascular Endothelial Growth Factor-A J. Immunol., April 15, 2004; 172(8): 5034 - 5040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Russo, B. Bussolati, I. Deambrosis, F. Mariano, and G. Camussi Platelet-Activating Factor Mediates CD40-Dependent Angiogenesis and Endothelial-Smooth Muscle Cell Interaction J. Immunol., November 15, 2003; 171(10): 5489 - 5497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. W. Kim, H. M. Lee, T. H. Lee, C. Kang, H. K. Kleinman, and Y. S. Gho Extracellular Membrane Vesicles from Tumor Cells Promote Angiogenesis via Sphingomyelin Cancer Res., November 1, 2002; 62(21): 6312 - 6317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Masso-Welch, D. Zangani, C. Ip, M. M. Vaughan, S. Shoemaker, R. A. Ramirez, and M. M. Ip Inhibition of Angiogenesis by the Cancer Chemopreventive Agent Conjugated Linoleic Acid Cancer Res., August 1, 2002; 62(15): 4383 - 4389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-M. Ko, K. H. Seo, S.-J. Han, K. Y. Ahn, I.-H. Choi, G. Y. Koh, H.-K. Lee, M. S. Ra, and S.-Y. Im Nuclear Factor {kappa}B Dependency of Platelet-activating Factor-induced Angiogenesis Cancer Res., March 1, 2002; 62(6): 1809 - 1814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Gebarowska, A. W. Stitt, T. A. Gardiner, P. Harriott, B. Greer, and J. Nelson Synthetic Peptides Interacting with the 67-kd Laminin Receptor Can Reduce Retinal Ischemia and Inhibit Hypoxia-Induced Retinal Neovascularization Am. J. Pathol., January 1, 2002; 160(1): 307 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Bussolati, C. Dunk, M. Grohman, C. D. Kontos, J. Mason, and A. Ahmed Vascular Endothelial Growth Factor Receptor-1 Modulates Vascular Endothelial Growth Factor-Mediated Angiogenesis via Nitric Oxide Am. J. Pathol., September 1, 2001; 159(3): 993 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. PONCE, M. NOMIZU, and H. K. KLEINMAN An angiogenic laminin site and its antagonist bind through the {alpha}v{beta}3 and {alpha}5{beta}1 integrins FASEB J, June 1, 2001; 15(8): 1389 - 1397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. B. DeRoock, M. E. Pennington, T. C. Sroka, K. S. Lam, G. T. Bowden, E. L. Bair, and A. E. Cress Synthetic Peptides Inhibit Adhesion of Human Tumor Cells to Extracellular Matrix Proteins Cancer Res., April 1, 2001; 61(8): 3308 - 3313. [Abstract] [Full Text]
|
|
|
|
|
|
J. B. Wyckoff, J. E. Segall, and J. S. Condeelis The Collection of the Motile Population of Cells from a Living Tumor Cancer Res., October 1, 2000; 60(19): 5401 - 5404. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. V. Ngo, M. Gee, N. Akhtar, D. Yu, O. Volpert, R. Auerbach, and A. Thomas-Tikhonenko An in Vivo Function for the Transforming Myc Protein: Elicitation of the Angiogenic Phenotype Cell Growth Differ., April 1, 2000; 11(4): 201 - 210. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Montrucchio, E. Lupia, E. Battaglia, L. Del Sorbo, M. Boccellino, L. Biancone, G. Emanuelli, and G. Camussi Platelet-Activating Factor Enhances Vascular Endothelial Growth Factor-Induced Endothelial Cell Motility and Neoangiogenesis in a Murine Matrigel Model Arterioscler Thromb Vasc Biol, January 1, 2000; 20(1): 80 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Biancone, V. Cantaluppi, M. Boccellino, L. Del Sorbo, S. Russo, A. Albini, I. Stamenkovic, and G. Camussi Activation of CD40 Favors the Growth and Vascularization of Kaposi's Sarcoma J. Immunol., December 1, 1999; 163(11): 6201 - 6208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kakolyris, A. Giatromanolaki, M. Koukourakis, I. M. Leigh, V. Georgoulias, P. Kanavaros, E. Sivridis, K. C. Gatter, and A. L. Harris Assessment of Vascular Maturation in Non-Small Cell Lung Cancer Using a Novel Basement Membrane Component, LH39: Correlation with p53 and Angiogenic Factor Expression Cancer Res., November 1, 1999; 59(21): 5602 - 5607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Jadeski and P. K. Lala Nitric Oxide Synthase Inhibition by NG-Nitro-L-Arginine Methyl Ester Inhibits Tumor-Induced Angiogenesis in Mammary Tumors Am. J. Pathol., October 1, 1999; 155(4): 1381 - 1390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Dentelli, L. D. Sorbo, A. Rosso, A. Molinar, G. Garbarino, G. Camussi, L. Pegoraro, and M. F. Brizzi Human IL-3 Stimulates Endothelial Cell Motility and Promotes In Vivo New Vessel Formation J. Immunol., August 15, 1999; 163(4): 2151 - 2159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Brizzi, E. Battaglia, G. Montrucchio, P. Dentelli, L. Del Sorbo, G. Garbarino, L. Pegoraro, and G. Camussi Thrombopoietin Stimulates Endothelial Cell Motility and Neoangiogenesis by a Platelet-Activating Factor–Dependent Mechanism Circ. Res., April 16, 1999; 84(7): 785 - 796. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Ponce, M. Nomizu, M. C. Delgado, Y. Kuratomi, M. P. Hoffman, S. Powell, Y. Yamada, H. K. Kleinman, and K. M. Malinda Identification of Endothelial Cell Binding Sites on the Laminin {gamma}1 Chain Circ. Res., April 2, 1999; 84(6): 688 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bagheri-Yarmand, Y. Kourbali, A. M. Rath, R. Vassy, A. Martin, J. Jozefonvicz, C. Soria, H. Lu, and M. Crepin Carboxymethyl Benzylamide Dextran Blocks Angiogenesis of MDA-MB435 Breast Carcinoma Xenografted in Fat Pad and Its Lung Metastases in Nude Mice Cancer Res., February 1, 1999; 59(3): 507 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Malinda, M. Nomizu, M. Chung, M. Delgado, Y. Kuratomi, Y. Yamada, H. K. Kleinman, and M. L. Ponce Identification of laminin {alpha}1 and ß1 chain peptides active for endothelial cell adhesion, tube formation, and aortic sprouting FASEB J, January 1, 1999; 13(1): 53 - 62. [Abstract] [Full Text]
|
|
|
|
|
|
G. Montrucchio, A. Sapino, B. Bussolati, G. Ghisolfi, S. Rizea-Savu, L. Silvestro, E. Lupia, and G. Camussi Potential Angiogenic Role of Platelet-Activating Factor in Human Breast Cancer Am. J. Pathol., November 1, 1998; 153(5): 1589 - 1596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Silvagno, A. Follenzi, M. Arese, M. Prat, E. Giraudo, G. Gaudino, G. Camussi, P. M. Comoglio, and F. Bussolino In Vivo Activation of met Tyrosine Kinase by Heterodimeric Hepatocyte Growth Factor Molecule Promotes Angiogenesis Arterioscler Thromb Vasc Biol, November 1, 1995; 15(11): 1857 - 1865. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Nomizu, W. H. Kim, K. Yamamura, A. Utani, S.-Y. Song, A. Otaka, P. P. Roller, H. K. Kleinman, and Y. Yamada Identification of Cell Binding Sites in the Laminin alpha1 Chain Carboxyl-terminal Globular Domain by Systematic Screening of Synthetic Peptides J. Biol. Chem., September 1, 1995; 270(35): 20583 - 20590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Corcoran, M. C. Kibbey, H. K. Kleinman, and L. M. Wahl Laminin SIKVAV Peptide Induction of Monocyte/Macrophage Prostaglandin E[IMAGE] and Matrix Metalloproteinases J. Biol. Chem., May 5, 1995; 270(18): 10365 - 10368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Mesri, H. J. Federoff, and M. Brownlee Expression of Vascular Endothelial Growth Factor From a Defective Herpes Simplex Virus Type 1 Amplicon Vector Induces Angiogenesis in Mice Circ. Res., February 1, 1995; 76(2): 161 - 167. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Grant, J. Kinsella, M. Kibbey, S LaFlamme, P. Burbelo, A. Goldstein, and H. Kleinman Matrigel induces thymosin beta 4 gene in differentiating endothelial cells J. Cell Sci., January 12, 1995; 108(12): 3685 - 3694. [Abstract] [PDF]
|
|
|
|
|
|
M. Santra, I. Eichstetter, and R. V. Iozzo An Anti-oncogenic Role for Decorin. DOWN-REGULATION OF ErbB2 LEADS TO GROWTH SUPPRESSION AND CYTODIFFERENTIATION OF MAMMARY CARCINOMA CELLS J. Biol. Chem., November 3, 2000; 275(45): 35153 - 35161. [Abstract] [Full Text] [PDF]
|
|