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JNCI Journal of the National Cancer Institute 1990 82(12):1055-1061; doi:10.1093/jnci/82.12.1055
© 1990 by Oxford University Press
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Journal of the National Cancer Institute, Vol. 82, No. 12, 1055-1061, June 20, 1990
© 1990 Oxford University Press

New Carbon Dioxide-Independent Basal Growth Medium for Culture of Diverse Tumor and Nontumor Cells of Human and Nonhuman Origin

David T. Vistica*, Dominic Scudiero, Philip Skehan, Anne Monks, Michael R. Boyd

Program Development Research Group, Developmental Therapeutics Program, Division of Cancer Treatment, National Cancer institute, Frederick Cancer Research Facility Frederick, Md
Program Resources, Inc., Frederick Cancer Research and Development Center Frederick, Md

*Correspondence to: David T. Vistica, Ph.D., Bldg. 1052. Rm. 121, Frederick Cancer Research Facility, Frederick, MD 21701-1013

A eukaryotic growth medium (Program Development Research Group Basal Growth Medium) was developed for CO2-independent maintenance and propagation of human and nonhuman tumor cell lines representing diverse histologies (e.g., cancers of the brain, colon, lung, ovary, and kidney, as well as leukemia and melanoma). It was also shown to be suitable for the maintenance and propagation of nontumor cells of human and nonhuman derivation. The medium derives its buffering capacity primarily from β-glycerophosphate, exhibits a stable physiologic pH of 7.3–7.4, and is optimized to facilitate growth in atmospheric CO2. It is also useful in cellular growth and cytotoxicity assays based on either the metabolic reduction of tetrazolium reagents or protein staining. The 50% inhibitory concentration values obtained with car-mustine, doxorubicin, and tamoxifen in cell lines maintained in the new medium under atmospheric CO2 were closely comparable to those obtained with these drugs against cells maintained in RPMI-1640 under a 5% CO2 environment. [J Natl Cancer Inst 82:1055– 1061, 1990]

Manuscript received September 5, 1989; revised April 11, 1990; accepted April 12, 1990.


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